Map Your Formulation Landscape
Labbot helps navigating the vast and intricate formulation landscape, enabling you to map critical stability zones with precision and confidence.
Leading Scientists & Labs are Using Labbot
"Labbot gives a big advantage when a systematic screening of physico-chemical conditions needs to be performed, both in the case of protein phase separation and drug release experiments.
Vito
Foderà
Navigating the formulation landscape for protein drugs is a complex and daunting journey.
Every protein is unique, and the path to stability is influenced by a maze of inter-connected factors.
Unpredictable ingredient interactions
Essential components like surfactants, preservatives, and stabilisers often interact in surprising ways. A stabiliser for one protein might destabilise another, creating unexpected detours in the formulation process.
Dynamic solution conditions
Variables like pH, ionic strength, and temperature act as shifting terrain, altering the behavior of proteins and additives and making the landscape even more difficult to chart.
Hidden zones of stability
Critical conditions for protein stability can remain buried—hidden peaks and valleys in the formulation landscape that traditional trial-and-error methods frequently overlook.
The Result
Countless hours spent testing combinations with no guarantee of success.
Labbot helps navigating the vast and intricate formulation landscape, empowering researchers to map critical stability zones with precision and confidence.
Control Key Variables
Effortlessly take control of key formulation factors like pH, temperature, and ionic strength, enabling you to chart unexplored areas and pinpoint conditions where stability thrives.
Track Critical Parameters
Monitor protein behavior in real-time, observing key indicators such as aggregation, unfolding, and molecular interactions to gain continuous insight into the formulation process.
Identify Zones of Stability
Go beyond traditional trial-and-error methods with precise, continuous measurements that uncover even narrowor hidden stability zones, leaving no part of the landscape uncharted.
Whether you’re developing a new therapeutic or refining an existing formulation, Labbot provides the clarity you need to make informed decisions faster and with greater confidence.
Phase Separation in Drugs
Insulin amyloid morphology is encoded in h-bonds and electrostatics interactions ruling protein phase separation
Samuel Lenton, Hussein Chaaban, Mohammed Khaled, Marco van de Weert, Birgit Strodel, Vito Foderà
Proteins can undergo liquid-liquid phase separation, forming dense droplets that serve as nucleation sites for amyloid fibril growth. This behavior is critical to understand for stable and effective drug formulations.
Labbot helps you:
- Visualize phase separation dynamics across varying conditions.
- Monitor droplet formation, dissolution, and transition in real-time.
- Uncover hidden behaviors using advanced light scattering and fluorescence techniques.
Determining insulin phase diagram as a function of different co-solutes
Detecting temperature dependent phase
separation of insulin.
Dissolution of protein dense phase in presence of excipients.
Stability of Protein Particles in Bio-Pharmaceutical Products
Disassembly and in vitro cell compatibility of α-lactalbumin particulates under physiologically relevant conditions
Mai Bay Stie, Dirk Fennema Galparsoro, Xin Zhou, Vito Foderà
Protein particles play a critical role in drug delivery, but their stability depends on a delicate balance of factors like particle maturation and dissolution. Understanding these processes is key to designing effective formulations.
Labbot helps you:
- Measure particle dissolution rates with precision.
- Track structural changes in real-time with light scattering and fluorescence.
- Gain deeper insights into particle stability for optimised drug release.
ThT fluorescence tracks particle internal structure
Light scattering tracks particle dissolution
The correlation between ThT fluorescence and light scattering reveals that particles dissolve by a different route based on maturity
Ready to Map Your Formulation Landscape?
Mapping Points of Interest
Modulating protein unfolding and refolding via the synergistic association of an anionic and a nonionic surfactant
Johanna Hjalte, Carl Diehl, Anna E. Leung, Jia-fei Poon, Lionel Porcar, Rob Dalgliesh, Helen Sjögren, Marie Wahlgren, Adrian Sanchez-fernandez
Protein stability often hinges on specific conditions within a vast parameter space. Identifying these critical zones is essential to guide formulation strategies effectively.
Labbot helps you:
- Pinpoint key stability zones across complex formulation landscapes.
- Scan variables like pH, ionic strength, and surfactant ratios with precision.
- Focus on regions that matter most for actionable insights.
Titrating the surfactant composition triggers conformational changes in the protein, that are tracked by tryptophan fluorescence
Regions of interest revealed by the fluorescence profiles are selected for further analysis with NMR to investigate the underlying interaction mechanisms.
Exploring Excipient Compatibility
Protein-Surfactant Interactions in Complex Systems: Study of Physical Stability of Proteins and Peptides
Johanna Hjalte
Excipients are vital for stability but can behave unpredictably, with interactions varying across conditions. Understanding these complexities is crucial for effective formulations.
Labbot helps you:
- Investigate excipient effects across a range of concentrations, pH, and temperatures.
- Identify stabilizing or destabilizing interactions with real-time monitoring.
- Gain clarity on the interplay between formulation components.
Evolution of scattering intensity during titration of 1 M NaCl to 10 mg/ml protein,and varying phenol concentration; 0.00 w% (orange), 0.36 w% (pink), 0.68 w% (green), 1.00 w% (blue)
Protein Characterisation
Data from the Labbot Lab
Mattias Törnquist
Every protein behaves uniquely under stress, and understanding these responses is vital for successful formulations. Probing aggregation, unfolding, and structural changes lays the foundation for stability.
Labbot helps you:
- Simultaneously track unfolding and aggregation dynamics.
- Explore the impact of stressors like temperature, agitation, and pH.
- Build a comprehensive profile to minimize unforeseen challenges.
Many proteins are sensitive to shear stress. This can easily be explored by tracking the formation of aggregates over time with and without active stirring.
Protein unfolding and aggregation can happen simultaneously or separately.
Probing both at the same time by measuring light scattering and protein fluorescence reveals the connection.
Protein unfolding and aggregation can happen simultaneously or separately.
Probing both at the same time by measuring light scattering and protein fluorescence reveals the connection.
Technologies
Proven Techniques – Reinvented & Automated
The measurement methods you trust. The sample control you need. Combined to answer the most challenging questions about your formulation landscape.
Standard Optical Cuvette
Fluorescence
Absorbance
Light Scattering
pH/Conductivity
Automatic Titrations
Temperature Control
Adjustable Mixing
Specifications
Base 12.5x12.5 mm. Z-height 8 mm
Capacity: 100 µl
Width: 21 cm
Height: 11 cm (23 cm with pump holder)
